Asiatic Acid Induces Mitochondrial Apoptosis via Inhibition of JAK2/STAT3 Signalling Pathway in Human Osteosarcoma

Osteosarcoma (OS), a severe malignant bone tumour, usually occurs in adolescents and children has poor prognosis. Asiatic acid (AA), an active component isolated from Centella asiatica (L.) Urb., exhibits appreciable anti-oxidant anti-tumour activities. So far, the effects underlying mechanisms of AA against OS have not been clarified. Here, we explored human involved mechanism mediating its actions. To evaluate on cell proliferation cells, viability colony formation assays were performed. Flow cytometry was used to apoptosis cells exposed mitochondrial membrane potential. Western blotting RT-PCR applied determine expression relevant proteins their mRNA levels. Our explorations showed that inhibits concentration- time-dependent manner, induces by intrinsic (mitochondrial) pathway. Importantly, found inhibition AA-induced phosphorylation JAK2/STAT3 signalling molecules decrease MCL-1 contributed efficacy AA. Collectively, our results suggest could evoke mitochondrial- induced suppression pathway expression. These strongly demonstrate be potential agent for treatment.